The content of this channel is made by the Enzyme Function Initiative (EFI) and is intended to provide advice on how to take advantage of the tools available at https://efi.igb.illinois.edu/. The EFI website contains a collection of webtools for creating and analyzing Sequence Similarity Networks (SSNs) and Genome Neighborhood Networks (GNNs). SSNs are opened, manipulated and analyzed in Cytoscape (https://cytoscape.org/).